Graphs to show the effects of inhibitors on enzyme action

1. Graphs to show the effects of Inhibitors on Enzyme Action Navigate your way though all of the animations clicking on

Inhibitors and Enzyme Action
GRAPHS TO SHOW THE EFFECTS OF
INHIBITORS ON ENZYME ACTION
WJEC GCE BIOLOGY
NAVIGATE YOUR WAY THOUGH ALL
OF THE ANIMATIONS CLICKING ON
EXPLANATIONS EACH TIME
THEN COMPLETE THE WORKSHEET

2. Normal enzyme reaction rate

Rate of reaction
Inhibitors and Enzyme Action
WJEC GCE BIOLOGY
NORMAL ENZYME REACTION RATE
1
Substrate concentration

3. Normal enzyme reaction rate

WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
NORMAL ENZYME REACTION RATE
Forward
Explaination

4. Normal enzyme reaction rate

Rate of reaction
Inhibitors and Enzyme Action
WJEC GCE BIOLOGY
NORMAL ENZYME REACTION RATE
2
1
Substrate concentration

5. Normal enzyme reaction rate

Forward
NORMAL ENZYME REACTION RATE
WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
Every active site is saturated. The
rate of the reaction is at its
highest.
Close
Explanation

6. Normal enzyme reaction rate

Rate of reaction
Inhibitors and Enzyme Action
WJEC GCE BIOLOGY
NORMAL ENZYME REACTION RATE
2
3
1
Substrate concentration

7. Normal enzyme reaction rate

Forward
NORMAL ENZYME REACTION RATE
WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
Every active site is
saturated, so there is no
increase in the rate of
reaction.
Close
Example

8. Normal enzyme reaction rate

NORMAL ENZYME REACTION RATE
Rate of reaction
WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
2
3
Revise
1
Competitive
Inhibitor
Substrate concentration

9. Competitive inhibitor reaction rate

COMPETITIVE INHIBITOR REACTION RATE
Rate of reaction
WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
2
Revise
1
Non-competitive
Inhibitor
Substrate concentration

10. Competitive Inhibitor example: An important enzyme in respiration is succinate dehydrogenase. Its substrate is succinate, which

COMPETITIVE INHIBITOR EXAMPLE:
AN IMPORTANT ENZYME IN RESPIRATION IS SUCCINATE
DEHYDROGENASE. ITS SUBSTRATE IS SUCCINATE, WHICH
IS CONVERTED INTO FUMARATE
Succinate
Inhibitors and Enzyme Action
A competitive inhibitor
binds to the enzyme’s
active site, and slows down
the process.
WJEC GCE BIOLOGY
Forward
Malonate
Close
Explanation
Succinate
dehydrogenase

11.

COMPETITIVE INHIBITOR EXAMPLE:
AN IMPORTANT ENZYME IN RESPIRATION IS SUCCINATE
DEHYDROGENASE. ITS SUBSTRATE IS SUCCINATE, WHICH IS
Forward
CONVERTED INTO FUMARATE
Succinate
WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
The substrate will soon find
an active site. The effect of
a competitive inhibitor is to
slow down the process.
Malonate
Close
Explanation
Succinate
dehydrogenase

12. Non competitive inhibitor

Rate of reaction
Inhibitors and Enzyme Action
WJEC GCE BIOLOGY
NON COMPETITIVE INHIBITOR
2
1
Re-Revise
Substrate concentration

13. Non competitive Example: Copper Sulphate and catalase

WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
NON COMPETITIVE EXAMPLE:
COPPER SULPHATE AND CATALASE
Forward
The shape of the active site
changes because the noncompetitive inhibitor has
bound to an allosteric site; so
preventing an enzymesubstrate complex from
forming = less products
formed
Hydrogen
peroxide
Close
Explanation
CuSO4
Catalase

14. Non competitive Example: Copper Sulphate and catalase

WJEC GCE BIOLOGY
Inhibitors and Enzyme Action
NON COMPETITIVE EXAMPLE:
COPPER SULPHATE AND CATALASE
Forward
Hydrogen
peroxide
The reaction rate cannot
reach the same point
because the noncompetitive inhibitors have
changed the shape of some
of the active sites.
Close
Explanation
CuSO4
Catalase