Tumor Irradiation

1.

Tumor Irradiation
MHC upregulation
Proinflammatory Cytokine release
Costimulatory molecules
upregulation
Upregulation of
LN homing receptors
Complement activation

2.

Principal APCs
Figure 2
Antigen types
Immunogenic treatments
HOCl oxidation
UV irradiation
Heat shock
hyperthermia
Synthetic encapsulation
Routes of
administration
CD123+
CD11cCD303+
CD304+
CD123CD11c+
CD141+
Clec9A+
CD123- CD16CD11c+ CD11c+
CD1c+
Clec9A-
CD19+
CD20+

3.

RNA (TMG)
Or long peptides
DNA extraction
WES
analyses
NGS
Mutations
calling
In silico peptide
prediction
T-cell
validation
Priming modalities
*
Blood collection and
tumor biopsy
Boosting
Synthetic vaccine
Personalized synthetic vaccine
Whole tumor vaccine
Patient
interrogation
Synthetic vaccine
Standard of care/ in situ approaches
Radiotherapy
Chemotherapy
Cryoablation
Immunomodulation
Synthetic vaccine

4.

Info for the next slide:
Proteins should look
like
On single color, where
the mutation/alteration
in another color, for
example:

5.

Altered proteome
Genomic alterations
Altered transcription
regulation and alternative
splicing
Missense
point mutation
Altered translation
Novel ORFs and readthrough
stop codons
Altered antigen processing
and presentation
e.g β2m mutations
polypeptides
Insertion or
deletion
Gene fusion
e.g. glycosylation
Alternative splicing
Here is a version of the figure for my part. I tried to modify it so that it will
not look so similar to the parental figure. If I get the polished figure in
PDF format, I will be able to make small adjustments.
Tumor specific exon
or intron retention
Protein overexpression