Evaluation of Tubocurarine ED50 on Electrically Stimulated Isolated Chick Biventer Cervicis Nerve-Muscle Preparation

1. Evaluation of Tubocurarine ED50 on Electrically Stimulated Isolated Chick Biventer Cervicis Nerve-Muscle Preparation

2. Assignment

Tubo.
2 mM
Contraction
(cm)
4.2
0.9
Wash
Tubo.
3 mM
2.2
Tubo.
4 mM
Wash
Tubo.
5 mM
Wash
Wash
Tubo.
8 mM
Tubo.
7 mM
Tubo.
6 mM
Wash
Wash

3. Assignment

Tubo.
2 mM
Contraction
(cm)
4.2
0.9
Wash
Tubo.
3 mM
2.2
Tubo.
4 mM
Wash
2.9
Tubo.
5 mM
Wash
3.3
Wash
3.6
Tubo.
8 mM
Tubo.
7 mM
Tubo.
6 mM
Wash
3.9
Wash
4

4.

% inhibition
Normal contraction
cm
Tubo. Conc.
µM
Suppression
of
contraction
4.2
2
0.9
0.9
4.2
×100 = 21.4 %
3
2.2
2.2
4.2
×100 = 52.3 %
4
2.9
2.9
4.2
×100 = 69 %
5
3.3
3.3
4.2
×100 = 78 %
6
3.6
3.6
4.2
×100 = 85.7 %
7
3.9
3.9
4.2
×100 = 92.8 %
8
4
4
4.2
×100 = 95.2 %

5.

100
92,8
90
E max
85,7
78
80
69
70
% inhibition
95,2
60
52,3
50
40
30
21,4
20
10
0
0
1
2
3
ED50
4
5
6
7
8
Tubo. Conc. µM
9