Practical pharmacology. Part 4. Dose response curve of acetylcholine

1.

Practical pharmacology
Part 4

2.

Dose response curve of acetylcholine

3.

Organ bath
Drum
Water bath

4. Conditions

Physiological solution
Temperature
Aeration

5. Our isolated organ

• Rabbit intestine
• Tyrode‘s solution
• Temperature 37˚C
• Aeration by air pump

6.

7.

8.

Ach
Ach
contraction

9.

10.

11.

12.

13.

Myogenic contraction
0.05
wash
0.1
0.2
0.3

14.

Now we need to draw the dose response

15.

5 cm
Peak
5 cm
4 cm
3 cm
Response
0.05
Base line
0.1
0.2
0.3

16.

Important note
0.05 ml
0.2 ml
0.1 ml
0.3 ml
Is volumes
We need to convert volumes into doses
?

17.

Conc. of ach.
0.1 g%
0.1 g
100 ml
x
0.1 ml

18.

x =
0.1 x 0.1
100
= 0.0001 g
= 0.1 mg

19.

Now draw your curve

20.

Response (cm)
7
6
x
5
x
4
3
x
x
2
1
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3

21.

Notes

22.

Response (cm)
7
6
x
5
x
4
3
x
x
2
1
This
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3

23.

Response (cm)
7
x
6
5
x
4
3
x
x
x
x
2
1
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3

24.

Response (cm)
7
x
6
5
x
4
3
x
x
x
x
2
1
0.05
0.1
0.15
0.2
Dose (mg)
0.25
0.3

25.

26.

27.

28.

29.

Complete your book

30.

Thank you