DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

1.

DNA interrogation by the CRISPR RNA-guided
endonuclease Cas9
Samuel H. Sternberg, Sy Redding, Martin Jinek, Eric C. Greene &
Jennifer A. Doudna
2014

2.

Martin Jinek, Jennifer A. Doudna
Berkeley, California

3.

CRISPR/Cas9 bacterial immune system

4.

1. Single-molecule visualization of Cas9
NIHMS555417-supplement-12.mov

5.

6.

1. Single-molecule visualization of Cas9

7.

2. Cas9-RNA finds targets by 3D diffusion
NIHMS555417-supplement-13.m4v

8.

3. A PAM is required for DNA interrogation

9.

4. Mechanism of RNA-DNA heteroduplex formation

10.

5. The PAM triggers Cas9 nuclease activity