(6 ECTS credits)
2. BIOE 321 Molecular BiologyCourse description:
• This course introduces the general concept of
biology covering topics like biological chemistry, cell
structure and function, metabolism and energy,
mitosis and meiosis, Mendelian genetics, genetics,
evolution, classification, biodiversity, prokaryotes
and eukaryotes, plant and animal systems, viruses,
ecology, cellular basis of development, cellular
differentiation and specialization.
• This course integrates lecture and laboratory
sessions throughout the semester.
3. BIOE 321 Molecular BiologyCourse objectives:
• This course explores molecular biology of
prokaryotic and eukaryotic cells through their
maintenance, expression, and evolution.
• DNA, RNA, and protein structure, DNA replication,
transcription, translation and regulation of gene
expression in eukaryotes and prokaryotes
• Various methods in molecular biology eg. Cloning,
RNA isolation and PCR, RLFP and cell culture.
4. Course ScheduleThursday (Lecture): 16.00-19:00 S-CSM.401
Monday (Lab section 1): 14.00-16.00 S-CSM-306
Tuesday (Lab section 2): 13:00-15:00 S-CSM-306
Friday (Lab section 3): 13:00-15:00 S-CSM-306
5. BIOE 321 Molecular BiologyCourse Learning Outcomes:
At the end of this course, students should be able to:
• Demonstrate a perspective on DNA, RNA, and protein structure.
• Make a comprehensive assessment of transcription, posttranscriptional modulation, and translation
• Explain how cloning is performed and basics of DNA replication.
• Describe the gene expression in prokaryotes and eukaryotes.
• Assess and report a laboratory experiment
Prerequisites: BIOE 222 Cell Biology.
6. BIOE 321 Molecular BiologyTextbook:
Molecular Biology, Robert F. Weaver. McGraw Hill Higher Education; 5th
edition (2011) ISBN-10: 0071316868
7. Online learning centerWebsite address:
8. BIOE 321 Molecular BiologyRequirements
• Attendance to the lecture must be at least 70% to pass the course.
• For repeat students, attendance to the lecture is mandatory and
lab section is facultative.
• Students who miss a lecture are completely responsible for obtaining
the material they missed.
• No make-up labs will be given.
• Excused absences must be documented their legitimacy is determined
by the instructor
• Late assignments will be reduced 20 % of its total for each day late,
not more than 3 days.
• Adherence to the University Academic Integrity policy is expected.
10. BIOE 321 Molecular BiologyTentative Lab Schedule
Cloning DNA in a plasmid
(RE digestion, Gel electrophoresis, gel purification,
Transformation, DNA ligation)
RNA work, gel electrophoresis (cDNA/RT-PCR)
(SDS PAGE gel, protein staining with commassie
Restriction fragment length polymorphism
Transfection–GFP construct (fluorescent)
Cell culture and flow cytometry