Molecular Biology

1.

BIOE 321
Molecular Biology
(6 ECTS credits)

2. BIOE 321 Molecular Biology

Course description:
• This course introduces the general concept of
biology covering topics like biological chemistry, cell
structure and function, metabolism and energy,
mitosis and meiosis, Mendelian genetics, genetics,
evolution, classification, biodiversity, prokaryotes
and eukaryotes, plant and animal systems, viruses,
ecology, cellular basis of development, cellular
differentiation and specialization.
• This course integrates lecture and laboratory
sessions throughout the semester.

3. BIOE 321 Molecular Biology

Course objectives:
• This course explores molecular biology of
prokaryotic and eukaryotic cells through their
maintenance, expression, and evolution.
• DNA, RNA, and protein structure, DNA replication,
transcription, translation and regulation of gene
expression in eukaryotes and prokaryotes
• Various methods in molecular biology eg. Cloning,
RNA isolation and PCR, RLFP and cell culture.

4. Course Schedule

Thursday (Lecture): 16.00-19:00 S-CSM.401
Monday (Lab section 1): 14.00-16.00 S-CSM-306
Tuesday (Lab section 2): 13:00-15:00 S-CSM-306
Friday (Lab section 3): 13:00-15:00 S-CSM-306

5. BIOE 321 Molecular Biology

Course Learning Outcomes:
At the end of this course, students should be able to:
• Demonstrate a perspective on DNA, RNA, and protein structure.
• Make a comprehensive assessment of transcription, posttranscriptional modulation, and translation
• Explain how cloning is performed and basics of DNA replication.
• Describe the gene expression in prokaryotes and eukaryotes.
• Assess and report a laboratory experiment
Prerequisites: BIOE 222 Cell Biology.

6. BIOE 321 Molecular Biology

Textbook:
Molecular Biology, Robert F. Weaver. McGraw Hill Higher Education; 5th
edition (2011) ISBN-10: 0071316868

7. Online learning center

Website address:
http://highered.mheducation.com/sites/0073525324/information_center_view0/index.html

8. BIOE 321 Molecular Biology

Requirements
• Attendance to the lecture must be at least 70% to pass the course.
• For repeat students, attendance to the lecture is mandatory and
lab section is facultative.
• Students who miss a lecture are completely responsible for obtaining
the material they missed.
• No make-up labs will be given.
• Excused absences must be documented their legitimacy is determined
by the instructor
• Late assignments will be reduced 20 % of its total for each day late,
not more than 3 days.
• Adherence to the University Academic Integrity policy is expected.

9.

BIOE 321 Molecular Biology

10. BIOE 321 Molecular Biology

Tentative Lab Schedule
Experiment
Subject
1
Cloning DNA in a plasmid
(RE digestion, Gel electrophoresis, gel purification,
Transformation, DNA ligation)
2
RNA isolation
RNA work, gel electrophoresis (cDNA/RT-PCR)
3
Protein
(SDS PAGE gel, protein staining with commassie
blue)
4
Restriction fragment length polymorphism
5* (optional)
Transfection–GFP construct (fluorescent)
6* (optional)
Cell culture and flow cytometry

11.

BIOE 321 Molecular Biology
Evaluation Type
Number
Percentage
Laboratory
4
25
Midterm
1
35
Final Exam
1
40
TOTAL
100