41.43M

Lephinix EZG EZG Benchtop Bioreactor PPT（EN）

1.

LePhinix EZG Benchtop
Bioreactor
Shanghai LePure Biotech Co., Ltd.
Kai
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

2.

2
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
2

4.

LePhinix EZG Benchtop Bioreactor
4
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

Application scenarios
• CHO、293、SF9、Vero、
E.coli、Pichia ---Culture
process development
• Process parameter
screening optimization
• Scale-up/down
Suspension
5
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
Adherence
Fermenter

6.

LePhinix EZG Pro
All operations are carried out on the central control
computer. Standard models can be equipped with 8
Vessel per computer
EZG Pro Twins
6
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
EZG Pro

7.

Vessel
Single layer vessel
2L
5L
10L
15L
customizable
Note All are working volumes
7
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

8.

Vessel
Jacket tank
2L
5L
10L
Note All are working volumes
8
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

9.

Vessel components
All components are made of 316L stainless steel
9
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

10.

02
Software

11.

Software
The software has intelligent
process management and
data analysis functions
Comply with 21 CFR PRAT 11,
GAMP5 and cGMP
requirements
Open development platform
to meet the needs of
personalized customization
Batch vessels can be
controlled simultaneously
Comes with Vessel integrity
test function
11
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
Comes with perfusion function

12.

Multiple control
Standard software licenses can
control up to 8 tanks, with
additional licenses or optional
server or cloud solutions if more is
needed.
12
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

13.

User management
Users can add and
delete users as required,
and define user
permission.
13
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

14.

15.

Peristaltic pump customize function
The peristaltic pump
can customize the
feeding process to cover
more R&D process
requirement.
15
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

16.

17.

18.

19.

20.

21.

22.

03
Cell Culture performance

23.

Comparison of culture effect with Sartorius
Data from customer

24.

Comparison of culture effect with Applikon
Data from customer

25.

26.

27.

04
Other USP Equipment

28.

Incubator
LePure Incubator has extremely precise temperature control and
temperature uniformity.
A lighter and stronger case was used, resulting in a significant
reduction in overall weight(200kg).
The total capacity is 302L, which can hold up to 104 125mL
shakers, 40 500mL shakers or 28 1000mL shakers.
Humidity control does not need water tank, can be equipped with
active humidifier for active humidity control.
28
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

29.

LePhinix™Rocking Bioreactor™
Parameter
Tray specification 10L 20L 50L
Replaceable tray
Culture volume 1-25L
Modular configuration
Perfusion function
LePhinix™Rocking Bioreactor Using disposable technology and cell culture technology, the cells can be
well expanded in a sterile environment
The equipment has flexible volume range, simple operation, precise and reliable control, easy process
amplification, can automatically control temperature, Air/O2/CO2/N2, dissolved oxygen, pH, shaking
Angle frequency, etc., can realize the perfusion function.
29
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

30.

LePhinix™ Rocking Bioreactor
Controller
7 “color capacitor screen or
23“ AIO computer
3MFC
Air: 0-1L/min
O2: 0-0.5L/min
CO2: 0-0.2L/min
Tray specification
Adaptation specification
2L 10、20、50L Rocking bag
Peristaltic pump
pH/DO
Optical pH sensor
Optical DO sensor
30
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
Waston Marlow 114DV*2
range of speed 0-150rpm

31.

LePhinix™ Single Use Bioreactor
High market recognition
60+ trial and 20+ delivery have
been completed
Commercial production delivery of
2000L grade has been completed
Consistent quality
LePure Bioreactor has accumulated
15,000 + hours of stable production
without failure
The mass production of reaction bag
has accumulated 200+ times without
leakage
Widespread application
The yield of CHO cell culture can reach
8g/L
There have been mature cases in
HEK293, SF9 and E.coli cell culture
It has been applied to artificial meat,
diagnosis and other special fields
31
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
LePhinix® LePhinix® LePhinix® LePhinix® LePhinix® LePhinix®
50L
200L
500L
1000L
2000L
M 50L
Max
50L
50L
200L
500L
1000L
2000L
Min
20L
22L
40L
100L
200L
400L
31

32.

Single Use Bioreactor bag
•Supports three modes: standardization,
simple customization, and deep
customization
32
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
Type
Specification
Application
BL
50-2000L
Suitable for LePure
bioreactor
BC
50-2000L
Suitable for Cytiva
bioreactor
BT
50-2000L
Suitable for Thermo
bioreactor
32

33.

LeKrius®Bioreactor Bag
Good security
The material passed rigorous biocompatibility test, the cell
culture performance was not affected, and the extract type
s and content were very low
•Excellent physical properties
High heat sealing strength, bending resistance and kneadin
g resistance guarantee bag integrity and high quality
Stable quality and supply
LeKrius® Membrane
Formula independent research and development, extrusio
n production process independent development and verific
ation, to ensure a complete and stable supply chain and q
33
uality control
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
33

34.

Single Use Bioreactor Bag-Tactics
•Background: Hengrui localization replacement
project
Cytiva 50L WAVE Thermo 500L SUB
Cytiva 50L
WAVE
Cytiva 500L XDR
Thermo 2000L SUB
Cytiva 2000L XDR
Hengrui production base in Suzhou has changed the imported bioreaction bags of 2000L production line into LePure disposable
biological reaction bags, and the actual effect after replacement is consistent with that of imported brands.
34
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
34

35.

Seed passage data
WAVE Passage
500L N-1
This figure shows the seed culture data of 13 batches of Thermo bioreaction bags and one
batch of LePure bioreaction bags. No other changes were made except for the bioreaction bags. It
can be seen from the line chart that Le Chun reaction bags have no effect on cell growth.
35
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.

36.

Production data
2000L VCD
2000L VIA
2000L Titer
36
External
© 2022 Shanghai LePure Biotech Co., Ltd. All Rights Reserved.
Those figures showing the 2000L culture data
of 13 batches of Thermo bioreaction bags and
one batch of LePure bioreaction bags. In
addition to the change of bioreaction, the
customer also made slight changes in the
feeding process. As can be seen from the line
graph, LePure bioreaction bag has no
negative effect on cell growth.

Lephinix EZG EZG Benchtop Bioreactor PPT（EN）

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

11.

12.

13.

14.

15.

16.

17.

18.

19.

20.

21.

22.

23.

24.

25.

26.

27.

28.

29.

30.

31.

32.

33.

34.

35.

36.

37.

38.

39.